THE IR SPECTROSCOPIC METHOD OF DIAGNOSTICS OF ENDOTOXEMIA IN CRITICAL CONDITIONS.ADVANTAGES AND PROSPECTS.
THE IR SPECTROSCOPIC METHOD OF DIAGNOSTICS OF ENDOTOXEMIA IN CRITICAL CONDITIONS.ADVANTAGES AND PROSPECTS.
Muradov A.M., Tuychiboeva M.N., Shumilina O.V.
THE IR SPECTROSCOPIC METHOD OF DIAGNOSTICS OF ENDOTOXEMIA IN CRITICAL CONDITIONS.ADVANTAGES AND PROSPECTS.
Department of Efferent Medicine and Intensive Care of the State Educational Establishment «Institute of Postgraduate Education in Health Sphere of Republic of Tajikistan»
Aim.By a method of molecular IR spectroscopy to receive the IR spectra of blood, depending on the degree of intoxication in patients with endotoxemia. Conduct a comparative analysis with other known methods from the viewpoint of efficiency, informativity, reliability, availability, affordability and to determine application prospects.
Materials and methods. Materials were blood samples of patients with endotoxemia which were exactly confirmed by conventional biochemical studies. Were determined indicators of toxicity of blood plasma: bilirubin, creatinine, leukocyte index of intoxication average molecules paramentsit test, necrotic bodies, circulating immune complexes, total and effective albumin concentration index of intoxication.
IR spectra were recorded using molecular infrared spectroscopy. Obtained data were compared with the standard measurements.
Results. In patients with endotoxemia is observed intensification of lipid peroxidation, immunosuppression, decreased antioxidant protection and detoxification ability of the organism. This is confirmed by corresponding changes in homeostasis and toxicity. Their dynamics are correlated with the stages of endogenous intoxication. IR spectra of healthy and diseased of endotoxemia significantly differ from each other in form, intensity, position of the peak frequency of the individual bands, relative to the stretching vibrations of -OH and -NH groups and to the stretching vibrations of -CH group and on the integral intensity. These changes are characteristic features and are distinguished between different nosologies in critical conditions and correlate with integral indicators of toxicity.
Conclusion. The method of molecular IR spectroscopy is the most efficient, non-traumatic, clear and informative. At diagnostics of endotoxicosis in critical conditions in the early stages of the disease consider it appropriate to supplement traditional biochemical research by data analysis of IR spectra of blood of patients.
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